Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific CCL5 (RANTES) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine RANTES (CCL5). Anti-Murine RANTES (CCL5)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine RANTES (CCL5) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine RANTES (CCL5) (500-P118) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine RANTES (CCL5).

Western Blot:
To detect mRANTES by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant mRANTES is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P118BT-1MG is provided as 2 × 500 µg.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide.
CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It induces histamine release from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It acts as a natural ligand for the chemokine receptor CCR5 and suppresses replication of R5 strains of HIV-1 that use CCR5 as a coreceptor.
Alternative splicing results in multiple transcript variants encoding different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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