Thermo Fisher Scientific CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The TM-b1 monoclonal antibody reacts with mouse CD122, the 90–110 kDa interleukin-2 receptor beta chain (IL-2Rβ). CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated upon activation. CD122 associates with CD25 and CD132 (common gamma chain) to form the high-affinity IL-2 receptor. TM-b1 inhibits high-affinity IL-2 binding.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry on mouse splenocytes at ≤1 µg/test (100 µL final volume). Cell count may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorochrome Information:
Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Recommended detection using a 450/50 bandpass filter or equivalent.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD122 (Interleukin-2 receptor beta chain) is involved in T cell-mediated immune responses, primarily expressed in the hematopoietic system. It exists in three forms:

• Low affinity: α subunit monomer (no signal transduction)
• Intermediate affinity: α/β heterodimer
• High affinity: α/β/γ heterotrimer

Intermediate and high-affinity forms mediate receptor endocytosis and mitogenic signaling. The CD122 gene encodes the β subunit, a type I membrane protein.
Associated diseases include oligoarticular juvenile idiopathic arthritis and rheumatoid factor-negative juvenile idiopathic arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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