Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PE, eBioscience

Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PE, eBioscience

Applications

• Reported Applications: Flow Cytometry (Flow)
• Tested Applications: Flow Cytometry (F9 cell line)
• Recommended Dilution: 5 µL (0.125 µg)/test

Publications

• View 19 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• eFluor 660
• PerCP-eFluor 710
• Custom conjugation available upon request

Product Specific Information

Description:
The eBioMC-480 (MC-480) antibody reacts with stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed on early mouse embryos, murine embryonal carcinoma cells (EC), embryonic stem cells (ES), and germ cells (EG) in both mouse and human.
No immunoreactivity is observed with undifferentiated human EC and ES cells. Differentiation of human EC increases SSEA-1 expression, while in mouse cells expression decreases.
SSEA-1 is also found on mature human granulocytes (CD15) and some monocytes, and is associated with cell adhesion, migration, and differentiation.

Applications Reported:
This antibody is validated for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested on F9 cell line for flow cytometry. Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.

Optical Properties:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:
Post-manufacturing filtered (0.2 µm).

Target Information

CD15 (Lewis X, Le(x); SSEA-1):
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and certain tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X.
Cells with high Le(x) expression show strong calcium-dependent self-aggregation, relevant to embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression is widely used for confirming Hodgkin’s disease diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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