Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PE, eBioscience
SSEA-1 항원을 인식하는 Mouse IgM 단클론 항체로, PE 형광 표지되어 있습니다. 인간 및 마우스 세포에서 반응하며, Flow Cytometry 분석에 최적화되어 있습니다. 세포 분화, 접착 및 이동 연구에 활용 가능합니다.
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Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PE, eBioscience
Applications
- Reported Applications: Flow Cytometry (Flow)
- Tested Applications: Flow Cytometry (F9 cell line)
- Recommended Dilution: 5 µL (0.125 µg)/test
Publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-480 (MC-480) |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10852715 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Biotin
- eFluor 660
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
Description:
The eBioMC-480 (MC-480) antibody reacts with stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed on early mouse embryos, murine embryonal carcinoma cells (EC), embryonic stem cells (ES), and germ cells (EG) in both mouse and human.
No immunoreactivity is observed with undifferentiated human EC and ES cells. Differentiation of human EC increases SSEA-1 expression, while in mouse cells expression decreases.
SSEA-1 is also found on mature human granulocytes (CD15) and some monocytes, and is associated with cell adhesion, migration, and differentiation.
Applications Reported:
This antibody is validated for flow cytometric analysis.
Applications Tested:
Pre-titrated and tested on F9 cell line for flow cytometry. Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Optical Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
Post-manufacturing filtered (0.2 µm).
Target Information
CD15 (Lewis X, Le(x); SSEA-1):
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types including granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and certain tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X.
Cells with high Le(x) expression show strong calcium-dependent self-aggregation, relevant to embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression is widely used for confirming Hodgkin’s disease diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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