Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PE, eBioscience
SSEA-4 항원을 인식하는 단클론 항체로, 인간 및 다양한 종의 줄기세포 연구에 적합합니다. PE 형광 표지로 유세포분석에 최적화되어 있으며, 고순도 친화 크로마토그래피 정제 제품입니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 14 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Mouse, Non-human primate, Rabbit |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG3 |
| Recommended Isotype Control | Mouse IgG3 Isotype Control (B10), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-813-70 (MC-813-70) |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11150238 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Biotin
- eFluor 660
- PerCP-eFluor 710
- Request custom conjugation available
Product Specific Information
The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein.
Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES), with decreased expression upon differentiation.
Recently, expression has also been found on bone marrow mesenchymal stem cells.
This antibody cross-reacts with mouse, rabbit, canine, chicken, and Old/New World monkeys.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated using the 2102Ep cell line at 5 µL (0.06 µg) per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen expressed along with SSEA-3, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, these markers are down-regulated during differentiation, while SSEA-1 is upregulated after retinoic acid-mediated differentiation.
In mouse stem cells, SSEA-1 expression decreases with differentiation, whereas SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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