Thermo Fisher Scientific CD338 (ABCG2) Monoclonal Antibody (5D3), PE, eBioscience
인간 ABCG2 단백질을 인식하는 5D3 클론 단클론 항체로, PE 형광표지되어 있습니다. Flow cytometry에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 줄기세포 및 약물 내성 연구에 유용하며, 4°C 암소 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 23 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Dog, Human, Mouse, Pig |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D3 |
| Conjugate | PE |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10854716 |
Product Specific Information
Description:
The 5D3 monoclonal antibody reacts with the extracellular portion of the human ABCG2 protein (also known as Bcrp1 and MXR). ABCG2, a member of the multi-drug resistance (MDR) family, is highly expressed in primitive side-population (SP) stem cells characterized by efflux of fluorescent dyes such as Rhodamine 123 and Hoechst 33342. In bone marrow, approximately 0.05% of cells show low fluorescence and are enriched for repopulating cells. SP cells expressing low or undetectable CD34 levels have been identified in multiple species, and ABCG2 expression appears highly conserved.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of ABCG2-transfected and peripheral blood cells. Recommended usage is 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration is recommended for specific assays.
Excitation/Emission:
Excitation: 488–561 nm; Emission: 578 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
ABCG2 (Breast Cancer Resistance Protein, BCRP1):
Also known as placenta-specific ABC transporter. Confers resistance to various chemotherapeutic agents including anthracyclines, mitoxantrone, bisantrene, and topotecan. Plays a protective role by removing toxins and regulating stem cell differentiation. Responsible for the side population (SP) phenotype and widely expressed in stem cells, placenta, liver, and intestine. May undergo N-linked glycosylation and dimerization in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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