Thermo Fisher Scientific NHERF-1 Polyclonal Antibody, Biotin
NHERF-1 단백질을 인식하는 Biotin 결합 폴리클로날 항체입니다. Western blot, ELISA, IP에 사용 가능하며, 사람, 생쥐, 랫트에 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, 4°C 또는 -20°C에서 보관 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:50,000 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids within region 255–305 on human Na(+)/H(+) exchange regulatory cofactor NHE-RF1 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5–1.5 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Proprietary buffer with 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NHERF1 (Na⁺/H⁺ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with β-catenin and is required for its localization at cell–cell junctions. It interacts with various G protein-coupled receptors and regulates their trafficking, as well as sodium–hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth. It contains a C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ domains, enabling head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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