Thermo Fisher Scientific NHERF-1 Polyclonal Antibody
NHERF-1 단백질 검출용 Thermo Fisher Scientific의 다클론 항체. Western blot, ELISA, IP에 적합하며 인간, 마우스, 랫트 반응성 보유. 항원 친화 크로마토그래피로 정제된 비결합형 액상 항체로, 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:50,000 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 255–305 of human Na(+)/H(+) exchange regulatory cofactor NHE-RF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Proprietary buffer with 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein associated with beta-catenin and required for its localization at cell-cell junctions. It interacts with various G protein-coupled receptors and regulates their trafficking, sodium-hydrogen exchange, and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth. It contains a C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ domains, enabling intramolecular regulation of its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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