Thermo Fisher Scientific NHERF-1 Polyclonal Antibody, FITC
FITC로 표지된 NHERF-1 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응. Western blot, ELISA, IP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제. 498/517 nm 형광 특성으로 세포 분석 및 단백질 상호작용 연구에 적합.
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Thermo Fisher Scientific NHERF-1 Polyclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:5,000 |
| ELISA | 1:50,000 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 255–305 of human Na(+)/H(+) exchange regulatory cofactor NHE-RF1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Proprietary buffer with 30% glycerol |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C avoiding freeze/thaw cycles; store in the dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50), is an adaptor protein that associates with beta-catenin and is required for its localization at cell-cell junctions. It interacts with various G protein-coupled receptors, regulating their trafficking and sodium-hydrogen exchange as well as sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and suppresses anchorage-independent growth. It contains a C-terminal ERM-binding region and two N-terminal PDZ domains, enabling head-to-tail intramolecular conformation for interaction regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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