Thermo Fisher Scientific TSC1 Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:3,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:100–1:1,000

Product Specifications

Product Specific Information

• Positive Control: 293T, A431, HeLa, HepG2, U87-MG, SK-N-SH, IMR32, SK-N-AS
• Predicted Reactivity: Rat (80%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases signaling of the mammalian Target of Rapamycin (TOR), a serine/threonine kinase regulating cell growth and the cell cycle.
TOR activity is stimulated by Rheb, a Ras superfamily G-protein, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity, suggesting that TSC1/TSC2 decreases Rheb-mediated TOR activation. Overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been identified, though its function remains unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일명: PA5-78208_TSC1_Q92574-1_Rabbit.svg, PA5-78208_TSC1_Q92574-1_Rabbit_PDP.jpeg)