Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 1.0 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 563 / 615 / 661 / 737
• Brilliant Violet 421 / 480 / 605 / 650 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue / Red / Yellow Series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 780
• Custom conjugation available

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper TCR assembly and surface expression. CD3 is expressed by thymocytes and all mature T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimal titration recommended.

Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm and is excited by a 405 nm violet laser. Ensure the cytometer supports this fluorochrome.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
This tandem dye is sensitive to light-induced oxidation. Protect vials and stained samples from light.

Fixation Compatibility:
Samples can be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)

Brilliant Violet™ 711 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 subunit complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are immunoglobulin superfamily members encoded on chromosome 11. They associate with cytoplasmic signaling molecules via tyrosine-based motifs.

• Present on 68–82% of normal peripheral blood lymphocytes and 65–85% of thymocytes
• Not expressed on B or NK cells
• CD3 gene defects are associated with CD3 immunodeficiency

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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