Thermo Fisher Scientific H4K5ac Recombinant Rabbit Monoclonal Antibody (SR31-07), ChIP-Verified
H4K5ac 표적에 특이적인 재조합 토끼 단클론 항체로, ChIP 검증 완료. 다양한 응용(WB, IHC, ICC, ChIP)에 최적화된 고감도 항체. 로트 간 일관성 및 높은 특이성 제공. 연구용으로만 사용 가능.
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Thermo Fisher Scientific H4K5ac Recombinant Rabbit Monoclonal Antibody (SR31-07), ChIP-Verified
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
| ChIP assay (ChIP) | 0.5–2 µg | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR31-07 |
| Immunogen | Synthetic peptide within Human Histone H4 (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809303 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Click here for Master Lot linking information.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer, around which ~146 bp of DNA is wrapped to form nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction.
The histone H4 gene is intronless, lacks polyA tails, and contains a palindromic termination element. It is located in the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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