Thermo Fisher Scientific H4K5ac Recombinant Rabbit Monoclonal Antibody (SR31-07), ChIP-Verified
H4K5ac 표적에 특이적인 재조합 토끼 단클론 항체로, ChIP 검증 완료. Western blot, IHC, ICC/IF, ChIP 등 다양한 응용 가능. 높은 특이성과 재현성, 동물 유래 성분 없는 포뮬레이션 제공. 장기 보관 시 -20°C에서 안정적 유지.
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- pk
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Thermo Fisher Scientific H4K5ac Recombinant Rabbit Monoclonal Antibody (SR31-07), ChIP-Verified
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
| ChIP assay (ChIP) | 0.5–2 µg | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SR31-07 |
| Immunogen | Synthetic peptide within Human Histone H4 aa 1–50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2866507 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening to select optimal clones for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the large rabbit immune repertoire
Click here for Master Lot linking information
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped, forming nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction.
The histone H4 gene is intronless, encodes a member of the histone H4 family, and is located in the large histone gene cluster on chromosome 6.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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