Thermo Fisher Scientific HSP70 Monoclonal Antibody (3A3), PerCP
HSP70 단백질을 검출하기 위한 PerCP 결합 단일클론 항체로, 다양한 생물 종에 반응합니다. Western blot, IHC, ICC/IF, IP 등 여러 응용에 적합하며, 단백질 안정화 및 스트레스 반응 연구에 유용합니다. 4°C 보관, Protein G 정제, 액상 형태입니다.
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Thermo Fisher Scientific HSP70 Monoclonal Antibody (3A3), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Immunoprecipitation (IP) | 2 µg |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Amphibian, Arthropod, Bacteria, Chicken, Fruit fly, Fish, Human, Mouse, Prawn, Rat, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3A3 |
| Immunogen | Human recombinant HSP70 overexpressed in E. coli |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931670 |
Additional Formats
Product Specific Information
0.2 µg/mL of MA5-45216 was sufficient for detection of HSP70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 70 kDa. May detect HSP70, HSC70, p75, and HSP72.
Target Information
HSP70 is a heat shock protein (HSP) expressed in response to various biological stresses, including high temperatures. The HSP70 family is highly conserved and induced by stress across organisms.
Human HSP70 family members include:
- HSP70: Strongly inducible and constitutively expressed in primate cells
- HSP72: Induced exclusively under stress conditions (72 kDa)
- HSC70: Constitutively expressed; involved in clathrin-coated vesicle uncoating
- GRP78 (BiP): ER-localized, glucose-regulated 78 kDa protein
- p75 (HSP75): Found in mitochondria
HSP70 stabilizes proteins against aggregation and aids folding of newly translated proteins. It also participates in the ubiquitin-proteasome pathway via interaction with AU-rich element RNA-binding protein 1.
The HSP70 gene is located in the MHC class III region, clustered with two related genes encoding similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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