Thermo Fisher Scientific CRYAB Monoclonal Antibody (3A10.C9), APC
CRYAB 단백질을 인식하는 마우스 모노클로날 항체로, APC 형광 표지되어 세포 분석에 적합합니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용 가능. Bovine, Human, Rat 반응성. 고순도 Protein G 정제, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3A10.C9 |
| Immunogen | Native Alpha B Crystallin |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931671 |
Additional Formats
Product Specific Information
0.5 µg/mL of MA5-45217 was sufficient for detection of 50 ng purified alpha B crystallin by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 20 kDa (predicted molecular weight ~21 kDa).
Does not cross-react with alpha A-crystallin, beta L-crystallin, beta H-crystallin, gamma-crystallin, HSP25, HSP27, or HSP47 proteins.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural function is to maintain the proper refractive index.
The mammalian lens contains three major classes of crystallins: alpha, beta, and gamma.
Alpha-crystallin is composed of alpha-A and alpha-B gene products and comprises about 40% of total lens protein.
It functions as a chaperone-like protein, preventing aggregation that may lead to cataract formation.
Phosphorylation of alpha-crystallin may occur in response to cellular stress and play a role in protein maintenance.
Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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