Thermo Fisher Scientific HSP70 Monoclonal Antibody (3A3), APC
HSP70 단백질을 검출하기 위한 Thermo Fisher Scientific의 APC 결합 단클론 항체. Western blot 및 면역세포화학(ICC/IF)에 적합. 다양한 종에 반응하며 높은 특이성과 재현성을 제공. 연구용으로만 사용.
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Thermo Fisher Scientific HSP70 Monoclonal Antibody (3A3), APC
Applications and Tested Dilution
- Western Blot (WB): 1:5,000
- Immunocytochemistry (ICC/IF): 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Amphibian, Arthropod, Bacteria, Chicken, Fruit fly, Fish, Human, Mouse, Prawn, Rat, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3A3 |
| Immunogen | Human recombinant HSP70 overexpressed in E. coli |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.46 mM phosphate / 2.48 mM MES, pH 7.2, with 2 mM EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931668 |
Additional Formats
Product Specific Information
0.2 µg/mL of MA5-45214 was sufficient for detection of HSP70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 70 kDa. May detect HSP70, HSC70, p75, and HSP72.
Target Information
HSP70 is a heat shock protein expressed in response to various biological stresses, including high temperatures. The HSP70 family includes highly conserved proteins such as HSP70, HSP72, HSC70, GRP78, and p75. These proteins aid in stabilizing and folding newly translated proteins and are involved in the ubiquitin-proteasome pathway. The HSP70 gene is located in the MHC class III region, clustered with two related genes encoding similar proteins.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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