Thermo Fisher Scientific Factor VIII Monoclonal Antibody (F8-2.2.32)

Applications

Western Blot (WB)

• Tested Dilution: 1–10 µg/mL

ELISA

• Tested Dilution: 1–10 µg/mL

Product Specifications

Product Specific Information

• MA1-10588 detects full-length human Factor VIII.
• This antibody does not cross-react with von Willebrand factor.
• Suitable for ELISA and Western blot applications.
• Immunogen: purified human Factor VIII.

Target Information

The Factor VIII gene encodes a coagulation factor that participates in the intrinsic pathway of blood coagulation. Factor VIII acts as a cofactor for factor IXa, which, in the presence of Ca²⁺ and phospholipids, converts factor X to its activated form Xa.
This gene produces two alternatively spliced transcripts:

1. Transcript variant 1 encodes a large glycoprotein (isoform a) that circulates in plasma and associates with von Willebrand factor in a noncovalent complex.
2. Transcript variant 2 encodes a smaller protein (isoform b) containing the phospholipid-binding domain essential for coagulant activity.

Defects in this gene cause hemophilia A, a common X-linked recessive coagulation disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.