Thermo Fisher Scientific HDAC3 Monoclonal Antibody (7G6C5)
HDAC3 단백질을 검출하기 위한 Mouse monoclonal antibody. WB, ICC/IF, ELISA, ChIP 등 다양한 응용에 적합. Human 시료에 반응하며, 약 48.8 kDa의 HDAC3 단백질을 인식. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
| ChIP assay (ChIP) | 4 µl/10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7G6C5 |
| Immunogen | Purified recombinant fragment of human HDAC3 (aa224–428) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at –20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10986336 |
Product Specific Information
MA5-15442 targets HDAC3 in indirect ELISA, IF, ChIP, and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human HDAC3 (amino acids 224–428) expressed in E. coli.
MA5-15442 detects HDAC3, which has a predicted molecular weight of approximately 48.8 kDa.
Target Information
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events.
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.
HDAC3 belongs to the histone deacetylase/acuc/apha family, possesses histone deacetylase activity, and represses transcription when tethered to a promoter.
It may regulate transcription through binding with the zinc-finger transcription factor YY1 and can down-regulate p53 function, thereby modulating cell growth and apoptosis.
This gene is regarded as a potential tumor suppressor gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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