Thermo Fisher Scientific Phospho-HICE1 pS70 (Ser70) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1.22 µg/mL

-

ELISA (ELISA)

1:17,000-1:45,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HICE1,uniProtId:Q9BT25-1,ncbiNodeId:9606,antigenRange:70,antigenLength:410,antigenImageFileName:600-401-C52_HICE1_Q9BT25-1_Rabbit.svg,antigenImageFileNamePDP:600-401-C52_HICE1_Q9BT25-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.22 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Hice1 pS70 affinity purified antibody is directed against the phosphorylated form of human Hice1 protein at the S70 residue. The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

Target Information

Hice1 contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.