Thermo Fisher Scientific Phospho-ATG13 (Ser318) Polyclonal Antibody
인간 ATG13 단백질의 인산화된 Ser318 잔기를 특이적으로 인식하는 친화정제 폴리클로날 항체입니다. Western blot, ELISA, Flow cytometry에 사용 가능하며, 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Phospho-ATG13 (Ser318) Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | 1:25,000–1:175,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-ATG13 pS318 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region near S318 of ATG13. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity-purified antibody is directed against the phosphorylated form of human ATG13 protein at the pS318 residue.
Reactivity occurs against human ATG13 pS318 protein and is specific for the phosphorylated form.
Minimal reactivity with non-phosphorylated ATG13 by ELISA and Western blot.
BLAST analysis indicates 100% sequence homology with human ATG13; cross-reactivity with other species is unknown.
Target Information
Autophagy is a process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, essential for normal growth control and cellular nutrient preservation under starvation.
ATG13 forms a complex with ULK1, ULK2, and FIP200, which is regulated by TOR phosphorylation.
Inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1, and ULK2, initiating autophagy.
Knockdown of ATG13 inhibits autophagosome formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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