Thermo Fisher Scientific Phospho-C-Myb (Thr486) Polyclonal Antibody
마우스 c-Myb 단백질의 Thr486 인산화형을 인식하는 고순도 폴리클로날 항체. Western blot 및 ELISA에 적합하며, 높은 특이성과 낮은 비특이 반응을 보임. -20°C 보관, 반복 동결-해동 방지 필요.
- 카탈로그번호
- 600-401-C47x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-C-Myb (Thr486) Polyclonal Antibody
Thermo Fisher Scientific Phospho-C-Myb (Thr486) Polyclonal Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:2,500
- ELISA: 1:5,000–1:16,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.91 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is specific for the phosphorylated form of mouse c-Myb protein at the pT486 residue. Reactivity occurs against mouse c-Myb pT486 protein and is minimal with non-phosphorylated forms by ELISA and western blot.
Reacts with endogenous c-Myb and SUMOylated overexpressed c-Myb in transfected Cos7 cells.
BLAST analysis indicates potential cross-reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee, as well as homologs from cattle and avian species.
Target Information
Transcriptional activator and DNA-binding protein that specifically recognizes the sequence 5'-YAAC[GT]G-3'. Plays a key role in regulating proliferation and differentiation of hematopoietic progenitor cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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