Thermo Fisher Scientific PARP1 Monoclonal Antibody (123)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

1:100-1:300

View 1 publication 1 publication

RNA Immunoprecipitation (RIP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Horse, Human, Mouse, Rhesus monkey, Rat

Published species

Dog, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

123

Immunogen

Recombinant protein derived from the C-terminal region of human PARP protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PARP1,uniProtId:P09874-1,ncbiNodeId:9606,antigenRange:1014,antigenLength:1014,antigenImageFileName:436400_PARP1_P09874-1_House_mouse.svg,antigenImageFileNamePDP:436400_PARP1_P09874-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2532215

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.