Thermo Fisher Scientific PARP1 Monoclonal Antibody (123)
PARP1 단백질을 인식하는 Mouse monoclonal antibody (Clone 123). Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. Protein A로 정제된 액상형 항체로, PBS buffer에 sodium azide 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 7 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 | - |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | View 1 publication |
| Flow Cytometry (Flow) | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:100–1:300 | View 1 publication |
| RNA Immunoprecipitation (RIP) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Horse, Human, Mouse, Rhesus monkey, Rat |
| Published Species | Dog, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 123 |
| Immunogen | Recombinant protein derived from the C-terminal region of human PARP protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2532215 |
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form, NAD) as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors. Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Asp216 and Gly217, a site conserved across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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