Thermo Fisher Scientific TRIM28 Polyclonal Antibody
TRIM28 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 인간, 생쥐, 랫드 반응성이 있으며 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 1 mg/mL 농도, 냉장 또는 냉동 보관 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1–2 µg/mL
Immunocytochemistry (ICC/IF)
- Tested Dilution: 5 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 14 amino acid peptide near the carboxy terminus of human TRIM28 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551837 |
Product Specific Information
- Suggested positive control: Human testis tissue lysate
- PA5-34485 can be used with blocking peptide PEP-1527
Target Information
The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and acts as a transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription via interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors to enhance repression efficiency and interacts directly with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 targets KRAZ1 to centromeric heterochromatin regions containing HP1alpha, contributing to transcriptional repression regulation. Mutant KAP-1 unable to bind HP1 leads to random KRAZ1 distribution and transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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