Thermo Fisher Scientific Phospho-Fascin (Ser39) Monoclonal Antibody (M315)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Product Specifications

Species Reactivity

Rat, Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M315

Immunogen

Clone (M315) was generated from a phospho-Fascin (Ser39) synthetic peptide (coupled to carrier peptide) corresponding to amino acids surrounding Ser39 in human Fascin. This sequence has high homology to the conserved site in rat and mouse Fascin, as well as to the conserved region in Fascin 2, but low homology to the conserved region in Fascin 3.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942608

Product Specific Information

The antibody detects a 55 kDa* band corresponding to fascin on SDS-PAGE immunoblots of mouse C2C12 cells treated with Calyculin A. This band is not observed after lambda phosphatase treatment.

Target Information

Fascin organizes filamentous actin into bundles with a minimum of 4. 1:1 actin/fascin ratio. It is probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.