Thermo Fisher Scientific Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Monoclonal Antibody (M132)

Applications

• Western Blot (WB): Tested dilution 1:1,000

Product Specifications

Product Specific Information

The antibody detects 46/50 kDa proteins corresponding to the apparent molecular mass of GSK-3β and GSK-3α on SDS-PAGE immunoblots of pervanadate-treated rabbit fibroblasts, as well as treated human SKN-SH and A431 cells.

Target Information

Glycogen synthase kinase-3 (GSK3) is a protein kinase originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. It regulates diverse cellular functions such as protein synthesis, cell proliferation, differentiation, microtubule assembly/disassembly, and apoptosis.
GSK3’s substrate specificity requires a pre-existing phosphoserine or phosphotyrosine four residues C-terminal to the phosphorylation site.
Two isoforms, GSK3α and GSK3β, are regulated by phosphorylation. Inactivation occurs via Ser9 (Ser21 in GSK3α) phosphorylation by PKB, while activation depends on Tyr216 (Tyr279 in GSK3α) phosphorylation.
Activated GSK3β phosphorylates multiple cellular proteins including p53, c-Myc, c-Jun, HSF-1, and cyclin D1, and is implicated in Alzheimer’s disease progression through tau phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.