Thermo Fisher Scientific IL-13 Monoclonal Antibody (eBio13A), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular Staining)
• Tested Dilution: ≤ 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• APC-eFluor 780
• Brilliant UV 805
• eFluor 450
• eFluor 660
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The eBio13A antibody reacts with mouse IL-13, a cytokine mainly produced by Th2 cells and antigen-primed CD8 T cells. IL-13 is involved in allergic inflammation, parasitic clearance, and cancer-related immune modulation. In humans, IL-13 regulates B cell isotype switching and down-modulates macrophage activity by reducing pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12).

Applications Reported:
Reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested:
Tested by intracellular staining of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended use ≤ 1.0 µg/test. Optimize antibody concentration empirically.

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility.

Staining Buffer Recommendation:
Use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize polymer dye interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Compatible with short-term methanol-based fixation (≤ 1 hour).

Excitation / Emission / Laser:
355 nm / 732 nm / Ultraviolet Laser.

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. It regulates inflammatory and immune responses by down-regulating macrophage activity and inhibiting pro-inflammatory cytokine production. IL-13 contributes to allergen-induced asthma through IgE-independent mechanisms, regulates B cell maturation and differentiation, and promotes IgE isotype switching. The IL-13 gene is part of a cytokine cluster on chromosome 5q, adjacent to IL4.

For Research Use Only. Not for use in diagnostic procedures.

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