Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-9D7), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Brilliant UV 395
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• eFluor 660
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Reported applications include intracellular staining followed by flow cytometric analysis.

This antibody has been pre-diluted and tested for intracellular staining of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00). Recommended usage is 5 µL (0.25 µg) per test, defined as staining a cell sample in a final volume of 100 µL (10⁵–10⁸ cells/test).

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is intended for use on cytometers equipped with a 355 nm ultraviolet laser. Ensure instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product #SB-4401) or Brilliant Stain Buffer (Product #00-4409) to minimize non-specific interactions.

Light Sensitivity: Protect vial and samples from light to prevent photo-induced oxidation.
Fixation: Samples may be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal brightness loss.
BUV737 is compatible with short-term methanol-based fixation (<1 hour).

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

IL-10 encodes a cytokine primarily produced by monocytes and lymphocytes. It regulates immune responses and inflammation by down-regulating Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It enhances B cell survival, proliferation, and antibody production, and inhibits NF-kappa B activity. IL-10 also plays a role in the JAK-STAT signaling pathway. Mouse knockout studies indicate its essential role in intestinal immunoregulation. Mutations in IL10 are linked to increased susceptibility to HIV-1 infection and rheumatoid arthritis. Related diseases include Graft-Versus-Host Disease and HIV-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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