Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific TNF alpha Monoclonal Antibody (MAb11), Brilliant Ultra Violet 737, eBioscience™

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 5 µL (1.0 µg)/test
Publications: Refer to Thermo Fisher citation database

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 660, 700
• APC
• Biotin
• Brilliant UV 661
• Brilliant Violet 421, 650
• eFluor 450
• FITC
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Custom conjugation available

Product Specific Information

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells, and CD4+ T cells. TNF alpha exhibits cytolytic activity against various tumor cells and plays a critical role in immune regulation. It exists in soluble (17 kDa) and membrane-bound (26 kDa) forms.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-diluted and tested using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended usage: 5 µL (1.0 µg) per test.

A test is defined as the amount of antibody that stains a cell sample in a final volume of 100 µL. Cell number may vary (10⁵–10⁸ cells/test).

Fluorophore Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm, excited by a 355 nm UV laser. Ensure cytometer compatibility.

When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to reduce non-specific interactions.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer or 1-step Fix/Lyse Solution.
Methanol Compatibility: Short-term methanol fixation is acceptable; avoid long-term storage in methanol-containing buffers.

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the TNF superfamily. Secreted mainly by macrophages, it binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR receptors. TNF alpha regulates immune responses, apoptosis, cell proliferation, lipid metabolism, and coagulation. It forms multimers (dimers, trimers, pentamers) and appears as a 17 kDa band under non-reducing SDS-PAGE conditions.

It plays roles in tumor necrosis, immune defense against pathogens, and inflammatory diseases such as autoimmunity, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지