Thermo Fisher Scientific COX2 Polyclonal Antibody
COX2 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간, 마우스, 랫트에 반응합니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용되며 염증 및 암 관련 COX2 연구에 유용합니다.
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Thermo Fisher Scientific COX2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 534–604 of Human COX2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.42 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7) with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2544714 |
Product Specific Information
- Recommended positive controls:
- THP-1: 100 ng/mL PMA (16 hr) + 1000 ng/mL LPS (24 hr)
- RAW264.7: 0.1 µg/mL PMA (16 hr) + 1 µg/mL LPS (24 hr)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
COX2 catalyzes the conversion of arachidonate to prostaglandin H2 (PGH2), a key step in prostanoid synthesis including inflammatory prostaglandins. This two-step reaction involves:
- Cyclooxygenase (COX) converting arachidonate to prostaglandin G2 (PGG2)
- Peroxidase reducing PGG2 to PGH2
COX2 is constitutively expressed in certain tissues (endothelium, kidney, brain) under physiological conditions and upregulated under pathological conditions such as cancer and inflammation. It is associated with increased cell adhesion, resistance to apoptosis, and tumor angiogenesis.
In cancer cells, COX2 promotes prostaglandin E2 (PGE2) production, influencing motility and proliferation. COX2 activity is naturally inhibited by calcitriol (active Vitamin D) and suppressed by glucocorticoids. It is also targeted by NSAIDs such as aspirin to reduce inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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