Thermo Fisher Scientific WIBG Monoclonal Antibody (OTI9C7), TrueMAB

Applications

• Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

WIBG is a key regulator of the exon junction complex (EJC), a multiprotein complex that binds immediately upstream of exon-exon junctions on mRNAs. It serves as a positional landmark for the intron-exon structure of genes and directs post-transcriptional processes such as mRNA export, nonsense-mediated mRNA decay (NMD), and translation.
WIBG acts as an EJC disassembly factor, enabling translation-dependent EJC removal and recycling. Its association with the 40S ribosomal subunit restricts its activity to translated mRNAs, preventing premature disassembly. It interferes with NMD and enhances translation of spliced mRNAs, likely by antagonizing EJC functions.
May bind RNA, though the in vivo relevance remains unclear. RNA-binding activity has been variably reported in literature (PubMed:14968132, PubMed:19410547).
[Source: UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.