Thermo Fisher Scientific UBE2D2 Monoclonal Antibody (OTI1D2), TrueMAB
UBE2D2 단백질을 인식하는 마우스 단클론 항체로, Western blot에 최적화되어 있습니다. E. coli에서 발현된 full-length 인간 UBE2D2 단백질로 면역화되었습니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 연구용으로만 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D2 |
| Immunogen | Full length human recombinant protein of human UBE2D2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The modification of proteins with ubiquitin is a key cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Ubiquitination involves three enzyme classes:
- Ubiquitin-activating enzymes (E1s)
- Ubiquitin-conjugating enzymes (E2s)
- Ubiquitin-protein ligases (E3s)
UBE2D2 encodes an E2 ubiquitin-conjugating enzyme that participates in the ubiquitination of the tumor suppressor protein p53, mediated by an E3 ligase.
Two alternatively spliced transcript variants encoding distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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