Thermo Fisher Scientific Protein S Monoclonal Antibody (8D5)

Applications

Tested Dilution

Publications

ELISA (ELISA)

1:4,000

-

Product Specifications

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

8D5

Immunogen

Protein S isolated from human plasma.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.92 mg/mL

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_1086923

Product Specific Information

HYB 232-11-02 has specificity for human protein S.

HYB 232-11-02 has been successfully used in ELISA procedures. HYB 232-11-02 reacts strongly with protein S. Strong reaction is seen in sandwich ELISA in combination with a biotinlabeled antibody against protein S. Reactivity is Ca++ dependent and is optimal when using a tris-buffer containing 1 mM CaCl2. In Dot blotting SDS-treatment abolishes the reactivity.

The HYB 232-11-02 immunogen protein S isolated from human plasma.

Epitope specificity differs from that of HYB 232-02.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Protein S is a vitamin K-dependent, potent neutral anticoagulant plasma glycoprotein. Protein S has a molecular weight of approximately 70 kDa and consists of a single polypeptide chain. The concentration of protein S in human plasma is 25 µg/mL, with a half-life of approximately two days. About 40% of Protein S in plasma is in a free form, whereas 60% is complexed with C4b-binding Protein (C4BP). Only the free Protein S functions as a cofactor to antigen presenting cells (APC) in the APC-dependent degradation of Factor Va and Factor VIIIa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.