Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), eBioscience
마우스 CD25(IL-2Rα) 인식 단일클론 항체로, T 및 B 세포 활성화 연구에 적합. Flow cytometry에 최적화되어 있으며, 고순도(>90%) 및 낮은 응집도(<10%)를 보장. 다양한 형광 표지 형식으로 제공되어 다중 분석에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 publication |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 201 publications |
| Neutralization (Neu) | - | 4 publications |
| Functional Assay | - | 7 publications |
| Inhibition Assays (IA) | - | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467176 |
Additional Available Formats
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 563 / 661 / 737 / 805
- Brilliant Violet 421 / 650 / 711 / 786
- eFluor 450 / 506
- FITC
- Functional Grade
- NovaFluor Blue 585 / 610-70S / 660-120S / 760
- Pacific Orange
- PE, PE-Cyanine5 / 5.5 / 7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. CD25 alone binds IL-2 with low affinity but forms a high-affinity receptor when associated with CD122 (IL-2Rβ) and CD132 (γ chain). The PC61.5 antibody inhibits IL-2 binding to both high and low affinity receptors, playing a role in lymphocyte differentiation, activation, and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes or Con A-stimulated splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration is recommended.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD25 (IL2RA) encodes the IL-2 receptor alpha chain, part of a heterotrimeric complex with IL2RB and IL2RG forming the high-affinity IL-2 receptor. Homodimeric IL2RA chains form low-affinity receptors, while IL2RB homodimers form medium-affinity receptors. IL2RA expression in mature thymocytes is monoallelic, regulating precise gene expression. IL2, mainly produced by mature T cells, functions as a growth, differentiation, and survival factor, stimulating B cell proliferation, NK cell activity, and inhibiting granulocyte macrophage colony formation. Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency and immune dysregulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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