Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugate Formats

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 660 / 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Custom conjugation available upon request

Product Specific Information

Description:
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (Integrin β1), a 110–120 kDa member of the β integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 forms heterodimers with α integrins (CD49a–f) to create VLA-1 through VLA-6 complexes and with CD51. These complexes mediate cell adhesion, migration, proliferation, and differentiation. CD29-containing integrins bind extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin, and can also interact with receptors like VCAM-1 and MadCAM-1.

Applications Reported:
Suitable for flow cytometry and immunoprecipitation. For functional assays, use Functional Grade purified eBioHMb1-1 (HMb1-1) (Product #16-0291-85).

Applications Tested:
Tested by flow cytometric analysis of mouse spleen, thymus, and bone marrow cells. Recommended ≤1 µg/test in 100 µL final volume. Optimize antibody concentration based on cell number (10⁵–10⁸ cells/test).

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

ITGB1 (Integrin Subunit Beta 1, CD29):
A 110 kDa cell surface glycoprotein expressed by various cell types including leukocytes and platelets. ITGB1 forms heterodimers with multiple α chains (α1–α6), determining binding specificity of β1 (VLA) integrins. It mediates adhesion to extracellular matrix proteins and is involved in immune cell trafficking, tissue remodeling, and inflammatory responses. Downregulation of ITGB1 has been linked to tumor cell drug resistance, while upregulation influences leukocyte migration and cytokine secretion in smooth muscle cells. Two major isoforms exist: β1A (ubiquitous) and β1D (cardiac and skeletal muscle).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.