Thermo Fisher Scientific NALP4 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1 µg/mL

Product Specifications

Product Specific Information

• A suggested positive control is K562 cell lysate.
• PA5-21020 can be used with blocking peptide PEP-1134.

Target Information

NALP proteins include the apoptosis regulator APAF1 (apoptotic protease activating factor 1) and mammalian NOD-LRR proteins, which are involved in inflammation and reproduction.
NALP4, also known as PYRIN-containing APAF1-like protein 4, has:

• A C-terminal leucine-rich repeat (LRR) region
• An N-terminal Pyrin domain (PYD)
• A NACHT domain and a NACHT-associated domain

In transfected 293 cells, NALP4 suppressed NF-kappa-B induction by TNF-α and IL-1-β, suggesting that NALP4 operates at a convergence point in these signaling pathways.
NALP4 also suppressed NF-kappa-B induction resulting from overexpression of several adapter proteins and kinases (TRAF2, TRAF6, RIP, IRAK2, IKK-α, and IKK-β), indicating its critical role in modulating NF-kappa-B activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.