Thermo Fisher Scientific HK2 Recombinant Rabbit Monoclonal Antibody (2H8L6)
HK2 단백질을 인식하는 재조합 토끼 단클론 항체로, WB 및 ICC/IF에 적합합니다. Expi293 시스템에서 발현되며 높은 특이성과 재현성을 제공합니다. 동물 유래 성분이 없는 포뮬레이션으로 일관된 품질을 유지합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–3 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–3 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2H8L6 |
| Immunogen | A recombinant protein corresponding to amino acids 325–444 of human HK2 (UniProt ID: P52789-1). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2532323 |
Product Specific Information
This antibody is predicted to react with bovine, mouse, and rat based on sequence homology.
Intact IgG appears on a non-reducing gel as a ~150 kDa band, and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Hexokinase (HK) catalyzes the phosphorylation of glucose to glucose-6-phosphate, the first step of glycolysis. There are four major isoenzymes: HK1, HK2, HK3, and HK4.
HK2 is predominantly expressed in insulin-responsive tissues such as skeletal muscle and is insulin-regulated. Studies in rats suggest HK2 contributes to enhanced glycolysis in rapidly growing cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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