Thermo Fisher Scientific Human HPRT1 (HGPRT) Endogenous Control (FAM/MGB probe, non-primer limited), 500 Reactions

Applied Biosystems™ Human HPRT1 (HGPRT) Endogenous Control (FAM™/MGB Probe, Non-Primer Limited)

The Applied Biosystems™ Human HPRT1 (HGPRT) Endogenous Control (FAM™/MGB Probe, Non-Primer Limited) is intended as an endogenous control. It enables relative gene expression quantification in cDNA samples when used with other gene expression assays.
Probe is labeled with 6FAM™ dye - MGB, and the primers are not limited. Can be used for singleplex PCR reactions.
Endogenous control is compatible with Inventoried and Non-Inventoried TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, and/or Custom TaqMan™ Primers and Probes.

Assay Details

TaqMan™ Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan™ Endogenous Controls as your controls to quantify gene expression.
This convenient collection of pre-designed probe and primer sets enables normalization of sample RNA or DNA amounts in a reaction.

Complete Solution for Quantitative Gene Expression

TaqMan™ Endogenous Controls simplify control selection for common human, mouse, rat, and eukaryotic genes, removing trial-and-error in assay design.

Simple to Use

All components are QC tested and formulated into a single 20X mix.
Compatible with universal conditions for two-step RT-PCR.
Use with TaqMan™ Universal PCR Master Mix (with or without AmpErase™ UNG) and cDNA samples to generate sensitive, reproducible, and quantitative gene expression data on Applied Biosystems instruments such as:

• 7900HT Real-Time PCR System
• 7300, 7500 Real-Time PCR Systems
• 7000 and 7700 Sequence Detection Systems

Flexible Offering

Each endogenous control uses proven 5′ nuclease chemistry.
Available configurations:

• FAM™ dye-labeled TaqMan™ MGB probe (250 nM final) + two unlabeled PCR primers (900 nM each)
• VIC™ dye-labeled TaqMan™ MGB probe (250 nM final) + two unlabeled PCR primers (150 nM each, primer limited)
• VIC™ dye-labeled TAMRA™ probe (250 nM final) + two unlabeled PCR primers (150 nM each, primer limited)

Choosing the Right Endogenous Control

Endogenous controls normalize target gene expression by correcting for differences in cDNA input.
For best results:

• Verify consistent expression across all samples.
• Ensure endogenous control expression is uniform.
• For multiplexing, the control gene expression level should exceed that of the target gene.

Multiplex vs. Singleplex PCR

• VIC™ dye-labeled controls are primer limited and suitable for multiplexing.
• FAM™ dye-labeled controls are not primer limited and intended for singleplex reactions.

For Research Use Only. Not for use in diagnostic procedures.

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