Thermo Fisher Scientific Porcine IL-6 Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Expression System

P. pastoris

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: EAEAERLEED. The EAEA portion showed incomplete cleavage of the alpha-Factor signal by the Ste 13 protease. Several amino acids were generated from the cloning vector. Purity is >95% by SDS-PAGE analyses.

Mature porcine IL-6 was expressed in Pichia pastoris, KM71H. GenBank Accession # P26893, amino acids 30 - 212.

Activity: The ED50 (or 1 unit of activity) of recombinant porcine IL-6 is typically 45 pg/mL. For in vitro applications. A dose-response assay should be performed to determine the optimal concentration for specific experiments.

Reconstitution and Dilution: Store lyophilized material at 4ºC. Reconstitute in distilled water to a concentration of 0.1 - 1 mg/mL. Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70ºC, avoiding multiple freeze-thaw cycles. PBS with 1% BSA or a neutral media containing <10% FCS are suitable buffers.

Endotoxin level is <0.05 EU/ug.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.