Thermo Fisher Scientific Human DLL4 Fc Chimera Recombinant Protein

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

HEK293 cells

Amino acid sequence

Human DLL4, amino acids Met1-Pro524 (Accession # NP_061947.1) with a C-terminal human IgG1 Fc region

Tag

Fc-tag

Molecular weight

100-110 kDa

Class

Recombinant

Type

Protein

Purity

≥95% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized Mouse NOTCH1-His at 2 µg/ml (100 µL/well) can bind human DLL4-hFc, the EC50 of human DLL4-hFc is 15-90 ng/mL. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1-8 µg/mL in the presence of 500 ng/mL recombinant human BMP2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.25 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (500 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.

Concentration is measured by UV.

Target Information

Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.