Thermo Fisher Scientific Human DLL4 Fc Chimera Recombinant Protein

Applications

ELISA (ELISA)

• Assay-dependent

Functional Assay (Functional)

• Assay-dependent

Product Specifications

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized Mouse NOTCH1-His at 2 µg/mL (100 µL/well) can bind human DLL4-hFc, the EC50 of human DLL4-hFc is 15–90 ng/mL.
2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1–8 µg/mL in the presence of 500 ng/mL recombinant human BMP2.

Product Specific Information

• It is recommended that the protein be aliquoted for optimal storage.
• Add 500 µL of sterile water to the vial to prepare a stock solution of 0.25 mg/mL.
• Concentration is measured by UV.

Target Information

Delta-like ligand 4 (DLL4), also known as Delta-4, is a homologue of the Drosophila delta protein and functions as a transmembrane ligand for the Notch1 receptor. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by VEGF as a negative feedback regulator to control angiogenic sprouting and promote the formation of a differentiated vascular network. DLL4 is strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 leads to increased vascular proliferation but defective maturation, resulting in reduced tumor growth without apparent toxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.