Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), APC-eFluor 780, eBioscience
마우스 CD8β 분자를 인식하는 단클론 항체로, APC-eFluor 780 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며 0.125 µg/test로 사용됩니다. 적색 레이저(633 nm)로 여기되며, 광에 민감하므로 암조건에서 보관해야 합니다.
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Thermo Fisher Scientific CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), APC-eFluor 780
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.125 µg/test
Publications: View 9 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), APC-eFluor™ 780, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioH35-17.2 (H35-17.2) |
| Conjugate | APC-eFluor™ 780 |
| Excitation / Emission Max | 756 / 785 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573943 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule.
CD8 beta associates with CD8 alpha to form the CD8 alpha/beta heterodimer expressed on thymocytes and peripheral cytotoxic T cells.
CD8 binds to MHC class I and plays a role in T cell development and activation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; ≤0.125 µg per test (100 µL final volume).
Cell number may range from 10⁵ to 10⁸ cells/test.
Careful titration recommended for optimal performance.
Fluorophore Information:
- Excitation: 633–647 nm
- Emission: 780 nm
- Laser: Red Laser
- Tandem dye sensitive to photo-induced oxidation — protect from light.
Fixation Guidance:
Samples can be stored in IC Fixation Buffer (Product # 00-8222)
or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes.
It mediates efficient cell-cell interactions and acts as a coreceptor with the T-cell receptor to recognize antigens presented by MHC class I molecules.
The functional coreceptor can be a homodimer (two alpha chains) or a heterodimer (alpha and beta chain).
Both chains share homology with immunoglobulin variable light chains.
This gene encodes CD8 beta chain isoforms; multiple alternatively spliced transcript variants exist.
A pseudogene is also located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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