Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), APC-eFluor 780, eBioscience

Thermo Fisher Scientific CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), APC-eFluor 780

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 3 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• Biotin
• Brilliant UV 737
• Brilliant Violet 650
• eFluor 450
• FITC
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBio124-1D1 monoclonal antibody reacts with human CD7 (gp40, Leu9), a 40 kDa receptor belonging to the immunoglobulin gene superfamily.
CD7 is expressed on immature and mature T lymphocytes, T cell leukemias, natural killer cells, and a small subset of normal and malignant B cells.
Cross-linking CD7 modulates T cell and NK cell activity, influencing calcium flux, adhesion molecule expression, cytokine secretion, and proliferation.
CD7 associates with phosphoinositol 3′-kinase, inducing D-3 phosphoinositides and tyrosine phosphorylation.

A clonogenic subset of human CD34(+) CD38(-) cord blood cells expressing CD45RA, HLA-DR, and high CD7 levels can generate B cells, NK cells, and dendritic cells, but not myeloid or erythroid cells.
CD7(+) phenotype distinguishes primitive lymphoid progenitors from pluripotent stem cells.
CD7 may cooperate with CD28 in Treg function, as CD28/CD7-deficient mice show reduced Treg numbers and suppressive activity.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.125 µg)/test.
One test = antibody amount to stain cells in 100 µL volume (10⁵–10⁸ cells/test).

Fluorochrome Information:
APC-eFluor 780 emits at 780 nm, excited by Red laser (633 nm).
Ensure instrument compatibility.
Light-sensitive; protect from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
Performance after fixation should be determined empirically.

Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily.
It contains an N-terminal region homologous to Ig kappa light chains and a proline-rich C-terminal stalk.
Expressed on T cells, NK cells, and some B cells, CD7 plays a role in T cell activation and signaling.
Expression of CD7 is a key marker in leukemia diagnostics.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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