Thermo Fisher Scientific CD5 Monoclonal Antibody (53-7.3), APC-eFluor 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 14 publications

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The 53-7.3 monoclonal antibody reacts with mouse CD5, a 67 kDa protein expressed by most thymocytes, mature T cells, and a subset of B cells. CD5 expression on B-1 cells defines a distinct lineage involved in T-B cell interactions.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test. Optimize titration for best results.

Fluorochrome Information:
APC-eFluor 780 emits at 780 nm and is excited by a 633 nm red laser. Ensure instrument compatibility.

Handling & Storage:

• Light-sensitive tandem dye; protect from light.
• Fixation: Samples can be stored using IC Fixation Buffer (#00-8222) or 1-step Fix/Lyse Solution (#00-5333) for up to 3 days at 4°C in the dark.
• Excitation: 633–647 nm; Emission: 780 nm; Laser: Red.
• Post-manufacturing filtration: 0.2 µm.

Target Information

CD5 is a 67 kDa single-chain transmembrane glycoprotein found on human T lymphocytes, most thymocytes, and subsets of activated B cells. It regulates T-cell proliferation and is expressed in many T-cell leukemias and lymphomas.
Diseases associated with CD5 dysfunction include thymus cancer and Richter’s Syndrome.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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