Thermo Fisher Scientific Phospho-PKC (Ser345) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to the epitope -EDR-S-KSAP- with a phosphorylated Ser 345 of human PKC if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKC,uniProtId:Q05513-1,ncbiNodeId:9606,antigenRange:345,antigenLength:592,antigenImageFileName:500-9244_PKC_Q05513-1_Rabbit.svg,antigenImageFileNamePDP:500-9244_PKC_Q05513-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

25mM tris with proprietary stabilizer

Contains

0.01% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Positive control: SKBR-3

Cellular location: Nuclear, cytoplasmic.

Target Information

Protein Kinase C (PKC) is a multifunctional protein kinase that plays a critical regulatory role in a wide variety of cellular signaling processes. It is an ubiquitous calcium and phospholipid-dependent protein kinase. At least 10 PKC isoforms are reported to exist which are classified as conventional, novel, and a typical isoforms (Ohno and Nishizuka, 2002). PKC is one of the three major protein kinases (including CaM Kinase II and PKA) that mediate cellular responses to second messengers. PKC regulates a number of cellular functions including neurotransmitter synthesis and release, gene expression, ion channel function and synaptic plasticity (Lu et al., 1999; Grosshans et al., 2002; Nishizuka, 1995). The catalytic fragment of PKC (PKC-M) does not require Ca2+ or phosphatidylserine for its activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.