Thermo Fisher Scientific NODAL Monoclonal Antibody (4H2)

Applications

Tested Dilution

Publications

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

4H2

Immunogen

NODAL (NP_060525.3, 180 a.a. approximately 279 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nodal,uniProtId:Q96S42-1,ncbiNodeId:9606,antigenRange:180-279,antigenLength:347,antigenImageFileName:H00004838-M12_Nodal_Q96S42-1_House_mouse.svg,antigenImageFileNamePDP:H00004838-M12_Nodal_Q96S42-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: CWPRPPTPPA TNVLLMLYSN LSQEQRQLGG STLLWEAESS WRAQEGQLSW EWGKRHRRHH LPDRSQLCRK VKFQVDFNLI GWGSWIIYPK QYNAYRCEGE

Target Information

NODAL is an important TGF-beta family protein in cellular differentiation. While constitutively expressed in many developing tissues, NODAL is problematically associated with the differentiation and development of many cancers. The expression of NODAL is regulated by a Notch signaling pathway, which when disrupted contributes to the aggressive behavior of metastatic cancer cells, particularly in melanoma. The abundance of NODAL expression in melanoma can help determine the stage of cancer and metastatic type/progression when tested in IHC. NODAL interactions with Cripto also predispose toward tumor progression. Overexpression of NODAL is also associated with preeclamptic placenta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.