Thermo Fisher Scientific CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), NovaFluor Red 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• NovaFluor Blue 660-120S
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1), a 42–45 kDa GPI-anchored protein structurally and functionally similar to CD38. CD157 is expressed on monocytes, macrophages, and bone marrow stromal cells. It acts as a pleiotropic ectoenzyme and receptor, involved in NAD⁺ metabolism and intracellular Ca²⁺ regulation. Upon ligand binding, CD157 can initiate phosphorylation of cytoplasmic proteins such as focal adhesion kinase (FAK).

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometry on human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test (100 µL total volume). Suitable for 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures designed to minimize spillover and spectral spread.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

Bone marrow stromal cell antigen 1 (BST1) is a GPI-anchored molecule facilitating pre-B cell growth. Also known as ADP-ribosyl cyclase 2 and DAMP-1, BST1 is expressed by the BST1 gene in humans. Its expression is enhanced in bone marrow stromal cells from rheumatoid arthritis patients, potentially contributing to abnormal B cell activation in this disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지