Thermo Fisher Scientific CD157 Monoclonal Antibody (eBioSY11B5 (SY11B5)), NovaFluor Yellow 590, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.4 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 610
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The eBioSY11B5 monoclonal antibody recognizes human CD157 (Mo5, BST-1), a 42–45 kDa GPI-anchored protein with structural and functional similarities to CD38. CD157 was initially identified for its expression on monocytes and macrophages and later found to be identical to BST-1, expressed on bone marrow stromal cells. It functions as both an ectoenzyme and receptor, involved in NAD⁺ metabolism and intracellular Ca²⁺ regulation. Upon ligand binding, CD157 can trigger phosphorylation of cytoplasmic proteins such as focal adhesion kinase (FAK).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested on normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Notes:
NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

CellBlox Plus Blocking Buffer:
Updated formulation (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.

• Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
• For single-color controls: use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Conjugate Technology:
Based on Phiton™ technology using nucleic acid dye structures engineered for minimal spillover and spread.

• Excitation: 552 nm
• Emission: 592 nm
• Recommended Laser: 561 nm (Yellow)

Target Information

Bone Marrow Stromal Cell Antigen 1 (BST1):
BST1 is a GPI-anchored molecule that supports pre-B cell growth. Also known as ADP-ribosyl cyclase 2 and DAMP-1, it is expressed by the BST1 gene in humans. BST1 expression is upregulated in bone marrow stromal cells from rheumatoid arthritis patients, potentially contributing to abnormal B cell activity in the disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지