Thermo Fisher Scientific HLA-DR Monoclonal Antibody (LN3), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 660, 700, Plus 488, Plus 647
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 480, 605, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436–780
• Request custom conjugation

Product Specific Information

The LN3 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class II molecule, HLA-DR.
HLA-DR is expressed on the surface of antigen-presenting cells (APCs) such as B cells, monocytes, macrophages, dendritic cells, and activated T cells.
It is a heterodimeric transmembrane protein composed of alpha and beta subunits, playing a key role in peptide presentation to CD4⁺ T lymphocytes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry of normal human peripheral blood cells
• Recommended Use: 5 µL (0.125 µg) per test in a final 100 µL staining volume
• Typical Cell Range: 10⁵–10⁸ cells/test

Brilliant Violet™ 421 (BV421) emits at 423 nm and is optimized for cytometers with a 405 nm violet laser.
For panels using multiple polymer dyes (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

• Excitation: 407 nm
• Emission: 423 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

HLA-DR is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and a 27 kDa beta chain (DRB).

• The DRA gene contains 5 exons encoding the leader peptide, extracellular domains, transmembrane region, and cytoplasmic tail.
• DRA acts as the sole alpha chain for DRB1, DRB3, DRB4, and DRB5, with DRB1 containing the polymorphisms that determine peptide-binding specificity.
• HLA-DR is expressed mainly on B lymphocytes, monocytes, macrophages, thymic epithelial cells, and activated T lymphocytes.
• The DR, DQ, and DP loci encode the major expressed products of the human MHC class II region.

These molecules bind processed peptides and present them to T-helper cells, initiating immune responses.
HLA and MHC antibodies are essential tools in Immunopeptidomics, aiding in neoantigen identification and characterization via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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