Thermo Fisher Scientific TrkB (extracellular) Polyclonal Antibody, Atto 488

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)RLEPNSIDPENITE, corresponding to amino acid residues 57-70 of rat TrkB, Extracellular domain if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TrkB,uniProtId:Q63604-1,ncbiNodeId:10114,antigenRange:57-70,antigenLength:821,antigenImageFileName:ANT-019-AG-50UL_TrkB_Q63604-1_Rabbit.svg,antigenImageFileNamePDP:ANT-019-AG-50UL_TrkB_Q63604-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Atto™ 488 Atto™ 488 Atto™ 488

Excitation/Emission Max

501/523 nm

Form

Lyophilized

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

NTRK2 (TRKB) belongs to the neurotrophic factor family of related polypeptides central to the development and maintenance of the mammalian nervous system. NTRK2 is the receptor for brain-derived neurotrophic factor (BDNF). Together, NTRK2 and BDNF regulate both short-term synaptic functions and long-term potentiation of brain synapses. TrkB is involved in the development and maintenance of the nervous system. Moreover, Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. TrkB is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through TrkB leads to cell differentiation. Mutations in the TrkB gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for the TrkB gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.