Thermo Fisher Scientific Amphiphysin Monoclonal Antibody (N8-2)
Amphiphysin 단백질을 표적으로 하는 Mouse IgG1 단클론 항체로, WB, IHC(P), IP에 사용 가능. 신경 말단의 시냅스 소포 재활용 연구에 적합하며, 양성 대조군으로 mouse 또는 rat brain 권장. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunoprecipitation (IP) | 2 µg |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N8-2 |
| Immunogen | Recombinant human GST-fragment protein (1–347AA) purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2538673 |
Product Specific Information
A suggested positive control for this product is mouse or rat brain.
Target Information
Amphiphysins are members of the BAR (Bin-Amphiphysin-Rvsp) protein superfamily, which play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs).
All BAR family members share a conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain.
Two isoforms of amphiphysin act together as a heterodimer and interact via the SH3 domain with dynamin, synaptojanin, and clathrin.
These complexes are involved in synaptic vesicle recycling at nerve terminals.
Amphiphysins are also known as dominant autoantigens in paraneoplastic Stiff-Man Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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