Thermo Fisher Scientific Amphiphysin Monoclonal Antibody (N8-2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunoprecipitation (IP)

2 µg

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

N8-2

Immunogen

Recombinant human GST-fragment protein (1-347AA) purified from E.coli if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Amphiphysin,uniProtId:P49418-1,ncbiNodeId:9606,antigenRange:1-347,antigenLength:695,antigenImageFileName:MA5-17205_Amphiphysin_P49418-1_House_mouse.svg,antigenImageFileNamePDP:MA5-17205_Amphiphysin_P49418-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2538673

Product Specific Information

A suggested positive control for this product is mouse or rat brain.

Target Information

Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein super family, play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs). All members of the BAR family share a highly conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain. Two isoforms of amphiphysin have been identified and act in concert as a heterodimer. Amphiphysins interact via its carboxy-terminal SH3 domain with dynamin, synaptojanin and clathrin. These complexes are implicated in synaptic vesicle recycling at nerve terminal. Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.