Thermo Fisher Scientific Collagen Type I Polyclonal Antibody
Rabbit polyclonal antibody specific to Collagen Type I from human and bovine sources. Suitable for WB, IHC, ELISA, and IP applications. High specificity with negligible cross-reactivity to other collagen types. Supplied as liquid, 1 mg/mL, affinity pur...
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| ELISA | 1:5,000–1:50,000 |
| Immunoprecipitation (IP) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Collagen Type I from human and bovine placenta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to opening. Product is stable at 4°C as undiluted liquid. Dilute only before immediate use.
For extended storage, mix with equal volume of glycerol, aliquot, and freeze at -20°C or below. Avoid freeze/thaw cycles.
Negligible cross-reactivity against other collagen types was detected by ELISA. Some anti-collagen antibodies may show reduced reactivity with denatured collagen or formalin-fixed, paraffin-embedded tissues.
This antibody reacts with most mammalian Type I collagens and shows negligible cross-reactivity with Type II–VI collagens. Non-specific cross-reaction with other serum or extracellular matrix proteins is negligible.
Target Information
Collagen I is a fibril-forming Type I collagen composed of two alpha-1 chains and one alpha-2 chain. It is abundant in bone, cornea, dermis, and tendon.
Mutations in the COL1A2 gene (encoding the alpha-2 chain) are linked to osteogenesis imperfecta types I–IV, Ehlers-Danlos syndrome type VIIB, classical Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.
Reciprocal translocations between chromosomes 17 and 22, involving Collagen Type I genes and PDGF-beta gene, are associated with dermatofibrosarcoma protuberans.
Two transcripts of Collagen I have been identified due to alternate polyadenylation signals.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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