Thermo Fisher Scientific Collagen Type I Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, mix with an equal volume of glycerol, aliquot contents, and freeze at -20°C or below. Avoid cycles of freezing and thawing.

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards.
Some class-specific anti-collagens may be specific for three-dimensional epitopes, which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin-embedded tissues.
This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V, or VI collagens.
Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Target Information

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain.
It is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis, and tendon.
Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I–IV, Ehlers-Danlos syndrome type VIIB, classical type, idiopathic osteoporosis, and atypical Marfan syndrome.
Symptoms associated with COL1A2 mutations tend to be less severe than those in COL1A1, reflecting different roles in matrix integrity.
Reciprocal translocations between chromosomes 17 and 22, where Collagen Type I genes and PDGF beta gene are located, are linked to dermatofibrosarcoma protuberans due to unregulated growth factor expression.
Two transcripts resulting from alternate polyadenylation signals have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.